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Originally Posted by Count Floyd 
Yeah, the Big Daddies weren't technically evil either.
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They weren't evil at all, really. It's been a while since I completed the game, but I can't remember any reference or action that would indicate any evil-doing. So there's a high chance that the Big Sister could be good.
OR, it could be that the player from the first game, Jack, turned Tenenbaum into a Big Sister, and made her go out to capture more little girls, for more harvesting. And the new person in the game could be someone trying to get to the bottom of the missing girl mystery, or a father/mother/brother character, looking for a child/sibling. This is all just conjecture, and possibly too easy an explanation. Also, I've had too much to drink.