Damn, I have to follow HAM. Again. Bastard.
TMP: The moment that sends chills down my spine is the first appearance of Kirk on Earth at Starfleet Command with Jerry Goldsmith's music reaching its peak.
But the best scene by far is when Kirk docks on the Enterprise. His look of awe and Scotty's smile seals the deal.
Wrath of Khan: Toss up between when Kirk rushes to Engineering and his final moments with Spock or David's talk with Kirk in his quarters where he says he's proud to be his son.
Search for Spock: I bought the DVD for the sequence where Kirk, Sulu, and Chekov break Bones out of jail and steal the Enterprise.
Voyage Home: Two lines. 1. "Well double dumbass on you." 2. "No I'm from Iowa I only work in outer space."
Final Frontier: The campfire scene and "Uh, excuse me. But what does God need with a starship?"
Undiscovered Country: The scene were Iman morphs into Kirk and he says "I can't believe I kissed you." to wit she replies "It must have been your life long ambition."
Also nice is when Bones says "You know I'd pay real money if he'd just shut up."
Generations: The saucer crash.
First Contact: The only thing in that movie that quailifies and just barely is the Dixon Hill holodeck scene when Picard goes off on the borg.
Insurrection: Any scene with Riker and Troi makes me yell out "It's about God damn time..."